Info-enriched characterization of mobile populations. Morphological information from pictures can add into the richness of flow cytometry facts. For instance, the determine displays an in any other case typical apoptosis assay making use of Annexin V and PI, introducing cell imaging to characterize cells in Every population to expose morphologically unique attributes. These insights couldn't are already obtained from multiplex staining on your own.
As samples are acquired over the Attune CytPix Flow Cytometer, the large-velocity brightfield digital camera captures and stores images of detected activities in a charge up to 6,000 visuals for each second, depending on the flow charge and picture measurement. For increased adaptability, Attune Cytometric Computer software permits you to modify the impression seize frequency as wanted and select a particular gate for impression seize, if required.
(B) RAW 264.7 macrophages will often be annotated inaccurately with respect to particle rely due to their complexity and noncircular borders. The number of singlets available for Assessment improves after the person trains and employs a more exact design.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating having a substantial-velocity brightfield digicam for simultaneous substantial throughput flow cytometry and superior resolution brightfield imaging.
"An image is just not pretty value 1000 dots, however it truly helps you to see photographs. It is excellent to find out pictures that correlate with staining. One example is, AnnexinV-beneficial cells which are morphologically distinctive than balanced cells, or CD14+ monocytes which are larger and even more textured than CD3+/CD4+/CD14- lymphocytes.
Additionally, it's illuminating to view the quantity of doublets slip through classic singlet gates, and terrific to confirm the standing of surprising double-good functions in my Examination."
Optimizing gating system. Even strong manual singlet gating is mistake-susceptible and remains a subjective conclusion place in Pretty much all flow cytometry assays. Imaging may be used to verify and change gates to incorporate only one cells of fascination.
Steady impression high-quality even more info at higher throughput Acoustic concentrating along with a large-velocity camera combine to picture these T cells consistently at reduced or large flow rates. Quickly modify concentration and digicam settings to fulfill experimental prerequisites.
The Attune NxT cytometer would be the reputable workhorse instrument your lab can trust in for consistent and trusted overall performance. The Attune CytPix model includes a substantial-velocity brightfield camera and AI-driven impression Assessment application, with latest updates like machine Finding out applications and an intuitive person interface, enabling Increased data precision, accuracy, and more quickly insights into sample biology.
Potentially most importantly, these activities have cells of Evidently various phenotypes which could lead on to incorrect conclusions about double constructive functions (particularly in scarce populations).
Audio waves empower precise cell alignment, causing Fantastic knowledge quality and ~10X a lot quicker sample flow charges.
Application Notice: Automatic impression Assessment cuts down person-to-user variability in flow cytometry gating tactics
Leverage the power of advanced technology and innovation to accelerate your discoveries. Have faith in in the Attune Flow Cytometer household to deliver the trustworthiness, speed, and accuracy that established your operate aside.
No clean/no lyse leukocyte Assessment with imaging. Cells were being obtained from 24-hour-aged blood by dilution in 1 mM EDTA ( Skilled UX/UI allows customers to teach AI products for expanded applications. (A) The “Teach Product” aspect provides annotation equipment and actual-time model education precision.
At the guts of every Attune Flow Cytometer lies our groundbreaking acoustic focusing engineering, which makes use of sound waves to meticulously align cells within the flow mobile. This modern method surpasses classic hydrodynamic techniques, causing speedier processing speeds, exceptional data high quality, and greater sample throughput.
The Attune Xenith cytometer is usually a high-parameter spectral analyzer, furnishing each spectral unmixing and standard flow abilities for detailed and versatile cell analysis.
Agilent Value Assure ensures you at least ten years of instrument use from your date you buy. Usually, We're going to credit rating you With all the residual price of the procedure toward an upgraded model.
What seemed to be a homogeneous population is in fact much more assorted—an insight that ought to be regarded when interpreting outcomes.